Obligate anaerobic bacteria were isolated and identified from 15 specimens of oral pyogenic infections and their susceptibility to 6 common antibiotics was determined by broth-disk method. All procedures including specimen collection, transport, culturing, and susceptibility testing were performed under optimal anaerobic conditions, utilizing Anaerobic Glove Box.
Total 32 strains of obligate anaerobic bacteria were isolated from 13 specimens, and 2 specimens yielded only facultative bacteria. The average number of anaerobic isolates was 2.6/specimen. The predominant isolates were gram-negative bacilli,. and the most prevalent species was Bacteroides melaninogenicus. Of 25 isolates tested for antibiotic susceptibility, 13 were resistant to penicillin G. All strains were susceptible to carbenicillin. Eubacterium lentum was resistant to 4 antibiotics (penicillin G, tetracycline, erythromycin, clindamycin), and Bacteroides fragile was resistent to 3 antibiotics (penicillin G, tetracycline, erythromycin).
|